Skin Skincare University

Peptide effective concentrations and the penetration challenge

LEVEL 3 Complete Guide to Peptides
KAIAN R&D Team | |

For peptides to work, they need adequate concentration and skin penetration — hurdles higher than expected.

Trace-amount signal molecules

Effective at 0.001% to 2% because they work through signals, not concentration.

The 500Da Rule

Below 500Da passes the stratum corneum more easily. Tripeptides (~300Da) and tetrapeptides (~400Da) penetrate well.

Palmitoylation

Fatty acid attachment increases lipophilicity for barrier passage.

Purity matters

99% vs 50% purity differ dramatically. Concentration disclosure is essential.

References

Key peer-reviewed sources behind the scientific statements in this article.

  1. Gorouhi F, Maibach HI. Role of topical peptides in preventing or treating aged skin. Int J Cosmet Sci. 2009;31(5):327–345. PubMed
  2. Bos JD, Meinardi MM. The 500 Dalton rule for the skin penetration of chemical compounds and drugs. Exp Dermatol. 2000;9(3):165–169. PubMed
  3. Robinson LR, Fitzgerald NC, Doughty DG, Dawes NC, Berge CA, Bissett DL. Topical palmitoyl pentapeptide provides improvement in photoaged human facial skin. Int J Cosmet Sci. 2005;27(3):155–160. PubMed
This article is reference information about cosmetic ingredients and does not guarantee efficacy. Figures and test results vary by condition.
← Back to Journal